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ytsJ [2019-08-08 13:13:01]
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ytsJ [2019-08-08 13:13:01]

malic enzyme, forms a transhydrogenation cycle with MalS for balancing of NADPH
Locus
BSU_29220
Isoelectric point
5.05
Molecular weight
43.51 kDa
Protein length
410 aa Sequence Blast
Gene length
1233 bp Sequence Blast
Function
malate utilization
Product
NADP-dependent malate dehydrogenase
Essential
no
E.C.
1.1.1.38
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    2,989,900 2,991,132

    Phenotypes of a mutant

  • Poor growth with malate as single carbon source PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • (S)-malate + NADP+ = pyruvate + CO2 + NADPH (according to Swiss-Prot) malate <- pyruvate

    • Protein family

  • malic enzymes family (according to UniProt)

    • Paralogous protein(s)

  • MalS, MleA

    • Modification

  • phosphorylated on Arg-27 PubMed

    • Cofactors

  • NADP+

    • Structure

  • 1WW8 (from Pyrococcus horikoshii, 46% identity, 63% similarity)

    • Localization

  • Cytoplasm (Homogeneous) PubMed

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    ytsJ
    Description
    PubMed
    view in new tab

    Genes
    dnaE-ytsJ
    Description
    PubMed

    Regulatory mechanism

  • LexA: repression, PubMed, in LexA regulon

    • Regulation

  • induced by DNA damage (LexA) PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A176 (ytsJ::erm), available at the NBRP B. subtilis, Japan
  • GP612 (spc), available in Jörg Stülke's lab
  • GP1143 (spc), available in Jörg Stülke's lab
  • BKE29220 (ytsJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCCTAAACACTCCTT, downstream forward: _UP4_TAATTTTAATTCATTCCAAA
  • BKK29220 (ytsJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCCTAAACACTCCTT, downstream forward: _UP4_TAATTTTAATTCATTCCAAA

    • GFP fusion

  • GP1432 (spc, based on pGP1870), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP1131 (spc, based on pGP1331), available in Jörg Stülke's lab PubMed

    • Labs working on this gene/protein

  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

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